Rapid Zymogram for Lipase

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A rapid assay for lipoprotein lipase.

A rapid assay for lipoprotein lipase activity employing a (14)C-labeled substrate is described. The method is very sensitive and suitable for routine use.

متن کامل

Rapid, reliable method for measuring serum lipase activity.

The method of Dirstine etal. [CLIN. CHEM. 14, 1097(1968)1has been modified to increase sensitivity and decrease manipulations. The modifications permit a 10-mm incubation, and a single analysis can be completed in 30 mm, or five concomitant assays can be done in an hour. Stability of the substrate has been increased by increasing the emulsifier concentration. Recovery of the fatty acid products...

متن کامل

Rapid assay for hormone-sensitive lipase activity of adipose tissue.

A highly specific and rapid assay for hormone-sensitive lipase activity of rat adipose tissue is described. The method employs emulsified 2,3-di-O-oleyl-[9,10-(3)H(2)]oleoyl glycerol as a substrate; it is very sensitive and is suitable for serial sampling.

متن کامل

Synthesis and Characteristics of Mesoporous Sol-gels for Lipase Immobilization

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

متن کامل

Rapid communication: nucleotide sequence of the ovine lipoprotein lipase cDNA.

Name of the Sequence. Ovine lipoprotein lipase cDNA. Genus and Species. Ovis aries. Origin of Clones. Because almost the whole 3′-untranslated region (3′UTR) was found to be lacking from the ovine lipoprotein lipase (LPL) cDNA sequence (1,656 bp including the 5′UTR, the coding sequence, and the first 44 bp of the 3′UTR) published by Edwards et al. (1993), we have undertaken to characterize this...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BioTechniques

سال: 1998

ISSN: 0736-6205,1940-9818

DOI: 10.2144/98245bm13